Hildreth W W, Avron M, Chance B
Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania.
Plant Physiol. 1966 Jun;41(6):983-91. doi: 10.1104/pp.41.6.983.
The kinetics of the 520 mmu absorption change in spinach chloroplasts and Chlorella vulgaris following a flash from the ruby laser have been determined as follows: rise halftime </= 0.3 x 10(-6) second; rapid recovery halftime = 5 to 6 x 10(-6) second; intermediate recovery halftime = 4 x 10(-4) second (spinach chloroplasts only); slow recovery halftime = 12 to 170 x 10(-3) second, dependent on the measuring light intensity and aerobicity of the suspension.The rapid phase of the 520 mmu reaction is approximately independent of temperature, from 295 degrees to 77 degrees Absolute.With increasing oxygenation of the sample, the extent of the rapid phase decreases, the extent of the slow phase increases, while the extent of the intermediate phase in spinach chloroplasts remains constant.In spinach chloroplasts, no recovery halftime of the 3 recovery phases for the 520 mmu absorption change was observed to correspond to the halftime for oxidation of cytochrome f (t((1/2)) = 1.3 x 10(-3) second).
已测定红宝石激光闪光后菠菜叶绿体和小球藻中520毫微米吸收变化的动力学如下：上升半衰期≤0.3×10⁻⁶秒；快速恢复半衰期为5至6×10⁻⁶秒；中间恢复半衰期为4×10⁻⁴秒（仅菠菜叶绿体）；缓慢恢复半衰期为12至170×10⁻³秒，取决于测量光强度和悬浮液的需氧性。520毫微米反应的快速阶段在295开尔文至77开尔文的温度范围内大致与温度无关。随着样品氧合作用的增强，快速阶段的程度降低，缓慢阶段的程度增加，而菠菜叶绿体中中间阶段的程度保持不变。在菠菜叶绿体中，未观察到520毫微米吸收变化的3个恢复阶段的恢复半衰期与细胞色素f氧化的半衰期（t(1/2)=1.3×10⁻³秒）相对应。
