Al-Ansari A A, Massoud M, Perry L A, Smith D S
Clin Chem. 1983 Oct;29(10):1803-5.
We developed polarization fluoroimmunoassays for 11-deoxycortisol in serum and saliva. To avoid interfering factors, the steroid was initially extracted from the biological fluids with dichloromethane. Assays could then be completed without any further separation procedures or need to correct for blank signals. The serum assay was suitable for following the response to the metyrapone test and results correlated acceptably with those by an established, specific, direct 125I-radioimmunoassay. The method is not sufficiently sensitive to detect 11-deoxycortisol in normal saliva, but greatly increased concentrations were found in post-metyrapone saliva and results agreed well with those by the radioimmunoassay as modified for salivary assay. Salivary 11-deoxycortisol assay would provide a convenient means of monitoring results of the metyrapone test.
我们开发了用于检测血清和唾液中11-脱氧皮质醇的偏振荧光免疫分析法。为避免干扰因素，首先用二氯甲烷从生物体液中提取该类固醇。然后无需任何进一步的分离程序或校正空白信号即可完成检测。血清检测适用于跟踪甲吡酮试验的反应，其结果与既定的、特异性的直接125I放射免疫分析法的结果具有可接受的相关性。该方法对检测正常唾液中的11-脱氧皮质醇不够敏感，但在甲吡酮试验后的唾液中发现其浓度大幅增加，且结果与经改良用于唾液检测的放射免疫分析法的结果吻合良好。唾液11-脱氧皮质醇检测将为监测甲吡酮试验结果提供一种便捷的方法。
