大肠杆菌的乳糖载体是否以单体形式发挥作用？
Does the lactose carrier of Escherichia coli function as a monomer?
作者信息
Wright J K, Weigel U, Lustig A, Bocklage H, Mieschendahl M, Müller-Hill B, Overath P
出版信息
FEBS Lett. 1983 Oct 3;162(1):11-5. doi: 10.1016/0014-5793(83)81039-4.
PMID:6352333
Abstract
The purified lactose carrier of Escherichia coli (product of the lac Y gene) is shown to be a monomer in detergent micelles of dodecyl-O-beta-D-maltoside. The negative-dominant phenotype of mutant carriers (lacY-d mutants) could not be verified by measurements of the rate of galactoside transport in lacY+/Y-d diploid strains. It is proposed that the membrane-embedded carrier functions as a monomer in galactoside-H+ symport.
摘要
大肠杆菌纯化的乳糖载体（lacY基因产物）在十二烷基 - O - β - D - 麦芽糖苷的去污剂胶束中显示为单体。通过测量lacY + / Y - d二倍体菌株中半乳糖苷转运速率，无法验证突变载体（lacY - d突变体）的负显性表型。有人提出，膜嵌入载体在半乳糖苷 - H + 同向转运中作为单体起作用。
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