D'Ardenne A J, Burns J, Sykes B C, Kirkpatrick P
J Pathol. 1983 Sep;141(1):55-69. doi: 10.1002/path.1711410107.
The distribution of fibronectin (FN) and type III collagen (IIIC) in normal adult human tissues have been directly compared using immunofluorescence and immunoperoxidase techniques on fresh frozen and formalin-fixed paraffin embedded material. Although in many tissues localisation of these two proteins appeared similar, two major differences were identified: (1) Where the ratio of extracellular matrix to cells is high (e.g. in breast, intestinal submucosa), FN was scanty and present predominantly in association with cells, whether of epithelial, endothelial or mesenchymal origin. IIIC was present diffusely throughout interstitial connective tissue. (2) In the highly specialised vascular beds of spleen and renal glomeruli, FN was abundant and accompanied by little or no IIIC. It is postulated that these differences reflect a generalised more intimate association of FN with cell surfaces and basal laminae which is not always discernible by light microscopy. Proximity of FN and IIIC may nevertheless be important for cell-matrix interactions. It was also noted that "reticulin" fibres as defined by silver impregnation do not all have an identical composition.
利用免疫荧光和免疫过氧化物酶技术，在新鲜冷冻及福尔马林固定石蜡包埋材料上，直接比较了正常成人组织中纤连蛋白（FN）和Ⅲ型胶原蛋白（IIIC）的分布情况。尽管在许多组织中这两种蛋白质的定位似乎相似，但仍发现了两个主要差异：（1）在细胞外基质与细胞比例较高的部位（如乳腺、肠黏膜下层），FN含量稀少，主要与上皮、内皮或间充质来源的细胞相关。IIIC则弥漫分布于整个间质结缔组织中。（2）在脾脏和肾小球高度特化的血管床中，FN丰富，而IIIC很少或几乎没有。据推测，这些差异反映了FN与细胞表面和基膜之间更普遍、更紧密的联系，而这种联系在光学显微镜下并不总是能分辨出来。然而，FN和IIIC的接近度对于细胞-基质相互作用可能很重要。还注意到，银浸染所定义的“网状纤维”并非都具有相同的组成。
