Reilly J T, Nash J R, Mackie M J, McVerry B A
J Clin Pathol. 1985 Aug;38(8):849-54. doi: 10.1136/jcp.38.8.849.
Forty six lymph nodes were examined with the indirect immunoperoxidase technique for the distribution of fibronectin and laminin. Fibronectin was present in the framework of the tissue and the basal lamina of blood vessels, giving a clear outline of nodal architecture. Intracellular fibronectin was observed in cases of reactive sinus histiocytosis, when about a third of macrophages exhibited strong positivity. Mast cells were positive. A pronounced increase in extracellular fibronectin was seen in nodular sclerosing Hodgkin's disease, although heavily hyalinised areas exhibited only superficial positivity. Reed-Sternberg and mononuclear Hodgkin's cells were consistently negative for fibronectin. Laminin staining was localised to vascular and marginal sinus basement membranes. No cellular positivity was evident. The distribution of laminin indicated a pronounced increase in vascularity in nodular sclerosing Hodgkin's disease, which was especially prevalent within the dense fibrous trabeculae. In contrast, however, examination of the other Rye subtypes showed a lesser degree of vascularity with numbers of vessels similar to those observed in reactive follicular hyperplasia. Laminin was found to be more efficient than factor VIII related antigen as a vascular marker.
采用间接免疫过氧化物酶技术对46个淋巴结进行检查，以观察纤连蛋白和层粘连蛋白的分布情况。纤连蛋白存在于组织框架和血管基底膜中，勾勒出淋巴结的清晰结构轮廓。在反应性窦组织细胞增生症病例中可观察到细胞内纤连蛋白，此时约三分之一的巨噬细胞呈现强阳性。肥大细胞呈阳性。在结节硬化型霍奇金病中可见细胞外纤连蛋白显著增加，不过重度玻璃样变区域仅表现为表面阳性。里德-斯腾伯格细胞和单核霍奇金细胞的纤连蛋白始终呈阴性。层粘连蛋白染色定位于血管和边缘窦基底膜。未发现细胞阳性。层粘连蛋白的分布表明结节硬化型霍奇金病血管明显增多，在致密纤维小梁中尤为常见。然而，相比之下，对其他赖氏亚型的检查显示血管化程度较低，血管数量与反应性滤泡增生中观察到的相似。结果发现，层粘连蛋白作为血管标志物比因子VIII相关抗原更有效。
