克隆的噬菌体T4 uvsW和uvsY基因在rec⁺和rec⁻大肠杆菌中的表达
Expression of cloned bacteriophage T4 uvsW and uvsY genes in rec+ and rec- Escherichia coli.
作者信息
DeVries J K, Wallace S S
出版信息
J Virol. 1983 Sep;47(3):406-12. doi: 10.1128/JVI.47.3.406-412.1983.
Abstract
Chimeric plasmids containing the uvsY uvsW region of the T4 genome were examined for the expression of these genes. Certain of these plasmids were shown to express the uvsY or the uvsW gene products by their ability to complement the UV sensitivity of infecting uvsW or uvsY mutant phage. Also, a chimeric plasmid containing both the uvsW and uvsY genes increases the survival of UV-irradiated, methyl methane sulfonate- or ethyl methane sulfonate-treated recA hosts.
摘要
对含有T4基因组uvsY uvsW区域的嵌合质粒进行了这些基因表达的检测。通过这些质粒补充感染uvsW或uvsY突变噬菌体的紫外线敏感性的能力，证明其中某些质粒可表达uvsY或uvsW基因产物。此外，含有uvsW和uvsY基因的嵌合质粒可提高经紫外线照射、甲基磺酸甲酯或乙基磺酸甲酯处理的recA宿主的存活率。
相似文献
1
Expression of cloned bacteriophage T4 uvsW and uvsY genes in rec+ and rec- Escherichia coli.克隆的噬菌体T4 uvsW和uvsY基因在rec⁺和rec⁻大肠杆菌中的表达
J Virol. 1983 Sep;47(3):406-12. doi: 10.1128/JVI.47.3.406-412.1983.
2
Function of cloned T4 recombination genes, uvsX and uvsY, in cells of Escherichia coli.克隆的T4重组基因uvsX和uvsY在大肠杆菌细胞中的功能。
Mol Gen Genet. 1988 Feb;211(2):350-6. doi: 10.1007/BF00330615.
3
Studies on the recombination genes of bacteriophage T4: suppression of uvsX and uvsY mutations by uvsW mutations.噬菌体T4重组基因的研究：uvsW突变对uvsX和uvsY突变的抑制作用
Genetics. 1987 Feb;115(2):219-27. doi: 10.1093/genetics/115.2.219.
4
Genetic analysis of dar, uvsW, and uvsY in bacteriophage T4: dar and uvsW are alleles.噬菌体T4中dar、uvsW和uvsY的遗传分析：dar和uvsW是等位基因。
J Virol. 1984 Dec;52(3):1028-31. doi: 10.1128/JVI.52.3.1028-1031.1984.
5
Expression and function of the uvsW gene of bacteriophage T4.噬菌体T4的uvsW基因的表达与功能
J Mol Biol. 1990 Aug 5;214(3):643-56. doi: 10.1016/0022-2836(90)90283-R.
6
Recombination hotspots in bacteriophage T4 are dependent on replication origins.噬菌体T4中的重组热点依赖于复制起点。
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6043-7. doi: 10.1073/pnas.88.14.6043.
7
Isolation and characterization of conditional alleles of bacteriophage T4 genes uvsX and uvsY.噬菌体T4基因uvsX和uvsY条件等位基因的分离与鉴定。
Genetics. 1984 Aug;107(4):505-23. doi: 10.1093/genetics/107.4.505.
8
An analysis of DNA repair and recombination functions of bacteriophage T4 by means of suppressors: the role of das.利用抑制因子对噬菌体T4的DNA修复和重组功能进行分析：das的作用
Virology. 1984 Sep;137(2):324-30. doi: 10.1016/0042-6822(84)90224-1.
9
Isolation and genetic characterization of new uvsW alleles of bacteriophage T4.噬菌体T4新uvsW等位基因的分离与遗传特征分析
Mol Gen Genet. 1990 Jul;222(2-3):257-64. doi: 10.1007/BF00633826.
10
Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli.克隆于大肠杆菌中的噬菌体T4的denV基因的表达。
Proc Natl Acad Sci U S A. 1981 May;78(5):2796-800. doi: 10.1073/pnas.78.5.2796.
引用本文的文献
1
Bacteriophage T4 genome.噬菌体T4基因组。
Microbiol Mol Biol Rev. 2003 Mar;67(1):86-156, table of contents. doi: 10.1128/MMBR.67.1.86-156.2003.
本文引用的文献
1
Mutants of phage T4 with increased sensitivity to ultraviolet.对紫外线敏感性增强的噬菌体T4突变体。
Virology. 1963 Jan;19:66-71. doi: 10.1016/0042-6822(63)90025-4.
2
Relationship between repair processes and mutation induction in bacteria.
Basic Life Sci. 1980;15:1-23. doi: 10.1007/978-1-4684-3842-0_1.
3
Rapid procedure for detection and isolation of large and small plasmids.用于检测和分离大小质粒的快速方法。
J Bacteriol. 1981 Mar;145(3):1365-73. doi: 10.1128/jb.145.3.1365-1373.1981.
4
Bacteriophage T4 gene transcription studied by hybridization to cloned restriction fragments.通过与克隆的限制性片段杂交研究噬菌体T4基因转录
J Mol Biol. 1980 Apr 15;138(3):423-45. doi: 10.1016/s0022-2836(80)80011-8.
5
Cloning of uvsW and uvsY genes of bacteriophage T4.噬菌体T4的uvsW和uvsY基因的克隆
Virology. 1982 Jul 15;120(1):122-9. doi: 10.1016/0042-6822(82)90011-3.
6
Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli.克隆于大肠杆菌中的噬菌体T4的denV基因的表达。
Proc Natl Acad Sci U S A. 1981 May;78(5):2796-800. doi: 10.1073/pnas.78.5.2796.
7
A restriction map of the bacteriophage T4 genome.噬菌体T4基因组的限制酶切图谱。
Mol Gen Genet. 1980;179(2):421-435. doi: 10.1007/BF00425473.
8
Superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid.细胞内猿猴病毒40脱氧核糖核酸的超螺旋密度异质性
J Virol. 1971 Jan;7(1):1-7. doi: 10.1128/JVI.7.1.1-7.1971.
9
Radiation-sensitive mutants of T4D. I. T4y: a new radiation-sensitive mutant; effect of the mutation on radiation survival, growth and recombination.T4D的辐射敏感突变体。I. T4y：一种新的辐射敏感突变体；突变对辐射存活、生长和重组的影响。
Mutat Res. 1969 Nov-Dec;8(3):431-9. doi: 10.1016/0027-5107(69)90060-8.
10
Recovery of UV-inactivated E. coli cells by the v-gene action of phage T4.
Mutat Res. 1968 Jul-Aug;6(1):175-9. doi: 10.1016/0027-5107(68)90115-2.
Zotero 插件