Baars A J, Breimer D D
Pharm Weekbl Sci. 1983 Aug 26;5(4):145-8. doi: 10.1007/BF01961471.
A rapid and selective determination of reduced glutathione in biological material is described, based on its conjugation with 1-chloro-2,4-dinitrobenzene. The reaction product has a UV absorption maximum at 340 nm and is analysed by reversed phase high pressure liquid chromatography. Linear calibration graphs were obtained in the concentration range between 50 microM and 2 mM glutathione in standard solutions and in biological material (rat liver and bacterial homogenates). The detection limit is about 2 microM glutathione when using 20 microliters injection samples.
本文描述了一种基于还原型谷胱甘肽与1-氯-2,4-二硝基苯结合的方法，用于快速、选择性地测定生物材料中的还原型谷胱甘肽。反应产物在340nm处有最大紫外吸收，并通过反相高压液相色谱进行分析。在标准溶液以及生物材料（大鼠肝脏和细菌匀浆）中，谷胱甘肽浓度在50μM至2mM范围内可得到线性校准曲线。当进样量为20微升时，检测限约为2μM谷胱甘肽。
