Maianskiĭ D N, Shcherbakova E G
Antibiotiki. 1983 Jul;28(7):521-6.
The principle of functional loading, i.e. stimulation of the neutrophiles with enterobacterial inactivated vaccine in vitro or with bacterial polysaccharides in vivo followed by the NBT-test is recommended for adequate estimation of the neutrophile link in the system of the host defense from infections. 97 practically healthy persons were examined in Novosibirsk and Norilsk and it was shown that in the regions of the Far North there were differences among the population with respect to the initial functional activity of the neutrophiles and especially with respect to their reaction to the stimulation. The effect of neutrophile stimulation in vitro with an inactivated vaccine of Serratia marcescens was higher and more stable than that with a single inhalation of prodigiosan. The prevalence of the least pathway providing increased resistance of the phagocytosing cells to infection in the population of the Far North might be the cause of a significant risk of infectious diseases in them.
为了充分评估宿主抗感染防御系统中的中性粒细胞环节，推荐采用功能负荷原理，即在体外使用肠杆菌灭活疫苗或在体内使用细菌多糖刺激中性粒细胞，随后进行硝基蓝四氮唑试验（NBT试验）。在新西伯利亚和诺里尔斯克对97名实际健康的人进行了检查，结果表明，在远北地区，人群中性粒细胞的初始功能活性存在差异，尤其是在对刺激的反应方面。用粘质沙雷氏菌灭活疫苗体外刺激中性粒细胞的效果比单次吸入灵菌红素的效果更高且更稳定。在远北地区人群中，提供吞噬细胞对感染抵抗力增强的最少途径的流行可能是他们患传染病风险显著的原因。
