Chien P, Rose L J, Schreiber A D
Immunol Commun. 1983;12(4):407-17. doi: 10.3109/08820138309050761.
We developed a method which provides a mechanism for isolating adherent mononuclear cells without subjecting them to traumatic physical or chemical methods of removal from their surface attachment sites. This method uses gelatin, which is solid at room temperature and liquid at 37 degrees C, as the adhering surface. Blood monocytes bind to gelatin-coated flasks at room temperature and are easily and gently removed when the gelatin is liquified at 37 degrees C. Monocytes, so isolated, are viable and functional [phagocytosis, adherence and Fc(IgG) and C3 receptor activity].
我们开发了一种方法，该方法提供了一种机制，可用于分离贴壁单核细胞，而无需让它们遭受从其表面附着位点去除时的创伤性物理或化学方法。此方法使用在室温下为固体而在37摄氏度时为液体的明胶作为附着表面。血液单核细胞在室温下与包被明胶的培养瓶结合，当明胶在37摄氏度液化时可轻松温和地被去除。如此分离出的单核细胞具有活力且功能正常[吞噬作用、黏附以及Fc（IgG）和C3受体活性]。
