Treves A J, Yagoda D, Haimovitz A, Ramu N, Rachmilewitz D, Fuks Z
J Immunol Methods. 1980;39(1-2):71-80. doi: 10.1016/0022-1759(80)90295-1.
A simple method for the isolation of human peripheral blood monocytes in cell suspension is described. The method is based on the adherence and detachment characteristics of monocytes during their in vitro cultivation and does not require any gradient centrifugation or modulating substances. The mean percentage of monocytes recovered from different donors was 81.9% (60-100%) of the original phagocytic cells found in the peripheral blood of the tested donors and the cell suspensions obtained consisted of more than 95% of monocytes. The monocytes isolated in suspensions show high activity in various monocyte functions tested.
本文描述了一种从细胞悬液中分离人外周血单核细胞的简单方法。该方法基于单核细胞在体外培养过程中的黏附与脱离特性，无需任何梯度离心或调节物质。从不同供体回收的单核细胞平均百分比为受试供体外周血中原始吞噬细胞的81.9%（60 - 100%），所获得的细胞悬液中单核细胞含量超过95%。悬浮分离得到的单核细胞在各项测试的单核细胞功能中表现出高活性。
