酿酒酵母孢子诱变程序。
Procedure for mutagenizing spores of Saccharomyces cerevisiae.
作者信息
Romano P, Soli M G, Suzzi G
出版信息
J Bacteriol. 1983 Nov;156(2):907-8. doi: 10.1128/jb.156.2.907-908.1983.
Abstract
A procedure for inducing mutants of a homothallic strain of Saccharomyces cerevisiae is described. The essential parts of the procedure are long incubation in Glusulase, which preferentially kills vegetative cells instead of spores, and treatment in 9% ethyl methanesulfonate, which also preferentially kills vegetative cells instead of spores. Consequently, the viable population is virtually 100% spores.
摘要
描述了一种诱导酿酒酵母同宗配合菌株突变体的方法。该方法的关键步骤是在葡糖酶中长时间孵育，葡糖酶优先杀死营养细胞而非孢子，以及在9%的甲磺酸乙酯中处理，甲磺酸乙酯同样优先杀死营养细胞而非孢子。因此，存活群体几乎100%是孢子。
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An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'.一种使用抗生素“制霉菌素”筛选营养缺陷型酵母突变体的富集方法。
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Genetical mutants induced by ethyl methanesulfonate in Saccharomyces.甲磺酸乙酯在酿酒酵母中诱导产生的遗传突变体。
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