Smith J A, Ngui-Yen J
J Clin Microbiol. 1983 Sep;18(3):509-11. doi: 10.1128/jcm.18.3.509-511.1983.
A total of 1,736 urine specimens were evaluated in a two-phase study, and the results were compared with those obtained by the standard semiquantitative culture plate method. In phase 1, 1,083 urine specimens were examined by utilizing modified broth containing 0.03% agar, whereas 653 specimens in phase 2 were examined after preincubation of the same broth overnight at 35 degrees C. In both phases of the study, more than one-half of the total urine samples (54%) were negative (less than or equal to 10(3) CFU/ml), and approximately 16% had colony counts of less than or equal to 10(5) per ml. The sensitivity and specificity in phase 1 were 99.5 and 70.5%, respectively, compared with 92.6 and 83.8% in phase 2. Preincubating the urine ampvettes at 35 degrees C reduced the false-positive rate from 21.9 to 10.5%. The performance requisites of acceptable sensitivity and specificity appeared to be met by the MS-2 (Abbott Laboratories, Diagnostics Div., Dallas, Tex.) screening method.
在一项分两阶段的研究中，共评估了1736份尿液标本，并将结果与通过标准半定量培养平板法获得的结果进行比较。在第一阶段，利用含有0.03%琼脂的改良肉汤检查了1083份尿液标本，而在第二阶段，653份标本在将相同肉汤于35℃预孵育过夜后进行检查。在研究的两个阶段中，超过一半的尿液样本（54%）为阴性（小于或等于10³CFU/ml），约16%的菌落计数小于或等于每毫升10⁵。第一阶段的敏感性和特异性分别为99.5%和70.5%，而第二阶段分别为92.6%和83.8%。将尿液安瓿在35℃预孵育可将假阳性率从21.9%降至10.5%。MS-2（雅培实验室，诊断部，得克萨斯州达拉斯）筛查方法似乎满足了可接受的敏感性和特异性的性能要求。
