Simpson A W, Boughton B J
J Immunol Methods. 1983 Oct 28;63(3):321-7. doi: 10.1016/s0022-1759(83)80005-2.
Previous bioassays for studying the opsonic regulation of the monocyte-macrophage system are subject to a variety of serious disadvantages which limit their value. An assay system is described which avoids many of these pitfalls and is applicable to the rapid assay of large numbers of samples. Human monocytes were isolated from peripheral blood and Saccharomyces cerevisiae used as the target particle. Particle ingestion was assessed using an electronic particle size analyzer and a close correlation was shown between this assay and a visual assessment of ingested micro-organisms.
以往用于研究单核细胞-巨噬细胞系统调理素调节的生物测定法存在各种严重缺陷，限制了其价值。本文描述了一种测定系统，该系统避免了许多此类缺陷，适用于大量样品的快速测定。从外周血中分离出人类单核细胞，并使用酿酒酵母作为靶颗粒。使用电子颗粒大小分析仪评估颗粒摄取情况，结果表明该测定法与对摄入微生物的视觉评估之间存在密切相关性。
