Weston W L, Dustin R D, Hecht S K
J Immunol Methods. 1975 Sep;8(3):213-22. doi: 10.1016/0022-1759(75)90114-3.
Monocyte-macrophages are required for the development of cell mediated immunity to a variety of microorganisms and tumors. Quantitative assays of human monocyte-macrophage function would be most useful in the evaluation of cell mediated immune function in man. Five quantitative assays are described that provide a human monocyte-macrophage function profile. These assays parallel the physiologic steps necessary for monocyte-macrophages to function as phagocytes: 1) chemotaxis, 2) opsonization, 3) phagocytosis, 4) phagocytosis-induced metabolic stimulation and 5) destruction of foreign material. Application of these quantitative assays will allow detection and dissection of disorders of monocyte-macrophage function in man.
单核细胞 - 巨噬细胞对于针对多种微生物和肿瘤的细胞介导免疫的发展是必需的。人类单核细胞 - 巨噬细胞功能的定量测定对于评估人类的细胞介导免疫功能最为有用。本文描述了五种定量测定方法，这些方法提供了人类单核细胞 - 巨噬细胞功能概况。这些测定方法与单核细胞 - 巨噬细胞作为吞噬细胞发挥功能所需的生理步骤平行：1）趋化作用，2）调理作用，3）吞噬作用，4）吞噬作用诱导的代谢刺激以及5）异物破坏。应用这些定量测定方法将有助于检测和剖析人类单核细胞 - 巨噬细胞功能障碍。
