富含脑脊液蛋白质的分离。方法学研究，第2部分。

Separation of cerebrospinal fluid-enriched proteins. A methodological study, Part 2.

作者信息

Wikkelsø C, Andersson M, Von Essen C, Rönnbäck L, Blomstrand C

出版信息

J Neurol Sci. 1983 Aug-Sep;60(3):419-29. doi: 10.1016/0022-510x(83)90153-3.

Abstract

Beta-trace protein, beta2-microglobulin and prealbumin were identified in a cerebrospinal fluid fraction containing proteins enriched in CSF-characteristic proteins obtained through affinity chromatography. The prealbumin amount was somewhat higher among CSF-enriched proteins from ventricular CSF than in the same fraction from lumbar CSF. Otherwise no variations in the CSF-enriched protein pattern were found in a ventriculo-spinal CSF-gradient analysed with isoelectric focusing. Affinity chromatography was done using insolubilized antisera against human serum proteins or against human cerebrum. Soluble brain proteins were analysed similarly and compared to the CSF-enriched protein pattern.

摘要

在通过亲和层析获得的富含脑脊液特征性蛋白质的脑脊液组分中，鉴定出了β-微量蛋白、β2-微球蛋白和前白蛋白。来自脑室脑脊液的富含脑脊液蛋白质中的前白蛋白含量，略高于来自腰段脑脊液相同组分中的前白蛋白含量。否则，在用等电聚焦分析的脑室-脊髓脑脊液梯度中，未发现富含脑脊液蛋白质模式的变化。使用针对人血清蛋白或人脑的不溶性抗血清进行亲和层析。对可溶性脑蛋白进行了类似的分析，并与富含脑脊液的蛋白质模式进行了比较。