The Key Laboratory of Industrial Biotechnology, Ministry of Education, Laboratory of Applied Microorganisms and Metabolic Engineering, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu Province, People's Republic of China.
World J Microbiol Biotechnol. 2012 Apr;28(4):1563-74. doi: 10.1007/s11274-011-0960-7. Epub 2011 Nov 26.
The effects of culture conditions on 2,3-butanediol (2,3-BD) production and its possible scale-up have been studied. A newly isolated Bacillus amyloliquefaciens B10-127, belonged to GRAS microorganisms and showed a remarkable 2,3-BD producing potency, was used for this experiment. Corn steep liquor, soybean meal and ammonium citrate were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration were further optimized via response surface methodological approach and determined to be 31.9, 22.0 and 5.58 g/l, respectively. The concentration of the obtained 2,3-BD increased significantly with optimized medium (62.7 g/l) when compared with unoptimized medium (45.7 g/l) and the 2,3-BD productivity was about 2.4-fold (The fermentation time was shorten from 72 to 42 h). To observe scale-up effects, batch fermentation was carried out at various working volumes. At a working volume of 20.0 l, the final 2,3-BD concentration and yield were 61.4 and 0.38 g/g at 36 h with a 2,3-BD productivity of 1.71 g/l h. This result shows similar amount of 2,3-BD obtained in lab-scale fermentation, and it is possible to scale up to larger fermentors without major problems.
已研究了文化条件对 2,3-丁二醇（2,3-BD）生产及其可能的放大的影响。根据 Plackett-Burman 实验设计的结果，使用属于 GRAS 微生物且具有显著 2,3-BD 生产能力的新分离的解淀粉芽孢杆菌 B10-127 进行了此实验。发现玉米浆、豆粕和柠檬酸铵是发酵的关键因素。通过最陡爬坡路径检查了这三个因素的最佳浓度范围，并通过响应面方法学方法进一步优化了它们的最佳浓度，分别为 31.9、22.0 和 5.58 g/l。与未优化的培养基（45.7 g/l）相比，优化后的培养基（62.7 g/l）使获得的 2,3-BD 浓度显著增加，2,3-BD 的生产力约提高了 2.4 倍（发酵时间从 72 小时缩短至 42 小时）。为了观察放大效果，在不同的工作体积下进行了分批发酵。在工作体积为 20.0 l 的情况下，在 36 h 时，最终的 2,3-BD 浓度和产率分别为 61.4 和 0.38 g/g，2,3-BD 生产力为 1.71 g/l/h。该结果表明，在实验室规模发酵中获得了相似量的 2,3-BD，可以在没有重大问题的情况下放大到更大的发酵罐中。
