Hens L, Baumann H, Cremer T, Sutter A, Cornelis J J, Cremer C
Exp Cell Res. 1983 Nov;149(1):257-69. doi: 10.1016/0014-4827(83)90397-x.
Chinese hamster cells (M3-1 line) in S phase were laser-UV-microirradiated (lambda, 257 nm) at a small site of the nucleus. Cells were fixed either immediately thereafter or in subsequent stages of the cell cycle, including prophase and metaphase. The microirradiated chromatin was visualized by indirect immunofluorescence microscopy using antibodies specific for UV-irradiated DNA. During the whole post-incubation period (4-15 h) immunofluorescent labelling was restricted to a small part of the nucleus (means, 4.5% of the total nuclear area). In mitotic cells segments of a few chromosomes only were labelled. Following microirradiation of chromosome segments in anaphase, immunofluorescent labelling was observed over a small part of the resulting interphase nucleus. A territorial organization of interphase chromosomes, i.e. interphase chromosomes occupying distinct domains, has previously been demonstrated by our group for the nucleus of Chinese hamster cells in G1. Our present findings provide evidence that this organization pattern is maintained during the entire cell cycle.
处于S期的中国仓鼠细胞（M3 - 1系）在细胞核的一个小区域接受激光紫外线微辐射（波长257 nm）。此后立即或在细胞周期的后续阶段（包括前期和中期）对细胞进行固定。使用针对紫外线照射DNA的特异性抗体，通过间接免疫荧光显微镜观察微辐射的染色质。在整个孵育后时期（4 - 15小时），免疫荧光标记局限于细胞核的一小部分（平均占总核面积的4.5%）。在有丝分裂细胞中，仅少数染色体的片段被标记。在后期对染色体片段进行微辐射后，在形成的间期细胞核的一小部分观察到免疫荧光标记。我们小组先前已证明G1期中国仓鼠细胞核中存在间期染色体的区域组织，即间期染色体占据不同的结构域。我们目前的研究结果提供了证据，表明这种组织模式在整个细胞周期中得以维持。
