Manzo C, Bianchin A, Pirozzi G, Totaro G
Oncology. 1983;40(6):395-9. doi: 10.1159/000225772.
Circulating immune complexes (CIC) of the IgG class were detected in sera from 37 (35.58%) out of 104 of the preoperated breast cancer patients studied using a CIq solid-phase enzymatic assay. Sera of positive patients were then tested to identify the IgG subclasses which are part of the complexes with the aim of showing a better correlation between CIC and disease evolution. IgG1- and IgG3-CIC were indirectly detected by the above test using monoclonal antibodies against the mentioned subclasses. Curves obtained with aggregated IgG1 and/or IgG3 showed good sensitivity and specificity of the test. Complexes containing both IgG1 and IgG3 were present in sera from the majority of patients; however, the relative amounts of these subclasses varied from patient to patient.
采用CIq固相酶法，在104例接受手术前的乳腺癌患者中，检测到37例（35.58%）患者血清中存在IgG类循环免疫复合物（CIC）。随后对阳性患者的血清进行检测，以确定构成复合物的IgG亚类，目的是显示CIC与疾病进展之间有更好的相关性。使用针对上述亚类的单克隆抗体，通过上述试验间接检测IgG1和IgG3 - CIC。用聚集的IgG1和/或IgG3得到的曲线显示该试验具有良好的敏感性和特异性。大多数患者血清中存在同时含有IgG1和IgG3的复合物；然而，这些亚类的相对含量因患者而异。
