de la Rosa M, Pérez M, Carazo C, Pareja L, Orts A, Cantudo P
Servicio de Microbiología, Hospital Virgen de las Nieves, Granada.
Microbiologia. 1994 Mar-Jun;10(1-2):181-6.
Streptococcus agalactiae, a Group B streptococcus, is the main cause of bacterial perinatal infection and is also an important opportunistic pathogen. Detection and identification of S. agalactiae are straight forward with special culture media, where Group B streptococci show a specific, typical pink or red pigment. To quickly and easily detect the pigment, culture media should contain: (i) starch; (ii) an inhibitor of the folate pathway; (iii) animal serum; (iv) a pepsic proteic hydrolysate; and (v) glucose, together with a high-capacity buffer. When selective antibiotics are added to culture media designed in this way, it is possible to detect S. agalactiae directly from clinical samples by observation of its pigment after less than 12 hours of aerobic incubation.
无乳链球菌，一种B族链球菌，是细菌性围产期感染的主要原因，也是一种重要的机会致病菌。使用特殊培养基对无乳链球菌进行检测和鉴定很简单，B族链球菌会呈现出特定的典型粉红色或红色色素。为了快速简便地检测该色素，培养基应包含：（i）淀粉；（ii）叶酸途径抑制剂；（iii）动物血清；（iv）胃蛋白酶蛋白水解物；（v）葡萄糖，以及一种高容量缓冲剂。当向以此方式设计的培养基中添加选择性抗生素时，通过在需氧培养不到12小时后观察其色素，就有可能直接从临床样本中检测出无乳链球菌。
