采用过氧化物酶-抗过氧化物酶法，用兔抗小鼠脑血清对骨髓单核细胞进行免疫电子显微镜标记。
Immunoelectron microscopy of the bone marrow mononuclears labelling with rabbit anti-mouse brain serum using peroxidase-anti-peroxidase method.
作者信息
Butenko Z A, Zak K P, Komissarenko S V, Gruzov M A, Khomenko B M
出版信息
Blut. 1983 Dec;47(6):343-9. doi: 10.1007/BF00320348.
Ultrastructural features of mouse bone marrow mononuclears (BMM) labelled with peroxidase-anti-peroxidase (PAP) complex using rabbit anti-mouse brain serum (RAMBS) and rabbit anti-mouse brain serum, absorbed with thymocytes (RAMBST) were investigated. After incubation of cells with RAMBST which have high activity against mouse pluripotent hemopoietic stem cells (CFUs) about 0.6% of BMM were labelled. According to their fine structure these cells were similar to those described earlier as presumptive pluripotent hematopoietic stem cells (PHSC). After incubation with RAMBS 1.9% of BMM were labelled, including cells marked by RAMBST and typical small lymphocytes with ultrastructure of T-lymphocytes.
研究了使用兔抗小鼠脑血清（RAMBS）和经胸腺细胞吸收的兔抗小鼠脑血清（RAMBST），通过过氧化物酶抗过氧化物酶（PAP）复合物标记的小鼠骨髓单核细胞（BMM）的超微结构特征。在用对小鼠多能造血干细胞（CFUs）具有高活性的RAMBST孵育细胞后，约0.6%的BMM被标记。根据其精细结构，这些细胞与先前描述为推定多能造血干细胞（PHSC）的细胞相似。在用RAMBS孵育后，1.9%的BMM被标记，包括被RAMBST标记的细胞和具有T淋巴细胞超微结构的典型小淋巴细胞。
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