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采用过氧化物酶-抗过氧化物酶法，用兔抗小鼠脑血清对骨髓单核细胞进行免疫电子显微镜标记。

Immunoelectron microscopy of the bone marrow mononuclears labelling with rabbit anti-mouse brain serum using peroxidase-anti-peroxidase method.

作者信息

Butenko Z A, Zak K P, Komissarenko S V, Gruzov M A, Khomenko B M

出版信息

Blut. 1983 Dec;47(6):343-9. doi: 10.1007/BF00320348.

DOI:10.1007/BF00320348
PMID:6360251
Abstract

Ultrastructural features of mouse bone marrow mononuclears (BMM) labelled with peroxidase-anti-peroxidase (PAP) complex using rabbit anti-mouse brain serum (RAMBS) and rabbit anti-mouse brain serum, absorbed with thymocytes (RAMBST) were investigated. After incubation of cells with RAMBST which have high activity against mouse pluripotent hemopoietic stem cells (CFUs) about 0.6% of BMM were labelled. According to their fine structure these cells were similar to those described earlier as presumptive pluripotent hematopoietic stem cells (PHSC). After incubation with RAMBS 1.9% of BMM were labelled, including cells marked by RAMBST and typical small lymphocytes with ultrastructure of T-lymphocytes.

摘要

研究了使用兔抗小鼠脑血清（RAMBS）和经胸腺细胞吸收的兔抗小鼠脑血清（RAMBST），通过过氧化物酶抗过氧化物酶（PAP）复合物标记的小鼠骨髓单核细胞（BMM）的超微结构特征。在用对小鼠多能造血干细胞（CFUs）具有高活性的RAMBST孵育细胞后，约0.6%的BMM被标记。根据其精细结构，这些细胞与先前描述为推定多能造血干细胞（PHSC）的细胞相似。在用RAMBS孵育后，1.9%的BMM被标记，包括被RAMBST标记的细胞和具有T淋巴细胞超微结构的典型小淋巴细胞。

相似文献

1
Immunoelectron microscopy of the bone marrow mononuclears labelling with rabbit anti-mouse brain serum using peroxidase-anti-peroxidase method.采用过氧化物酶-抗过氧化物酶法，用兔抗小鼠脑血清对骨髓单核细胞进行免疫电子显微镜标记。
Blut. 1983 Dec;47(6):343-9. doi: 10.1007/BF00320348.
2
[Ultrastructure of hematopoietic and stromal cells of the subendosteal region of the bone marrow].[骨髓骨内膜下区域造血细胞和基质细胞的超微结构]
Tsitologiia. 1985 Jun;27(6):628-32.
3
An immunologic comparison between bone marrow and spleen-derived pluripotent hemopoietic stem cells (CFU-S) of mouse : effect of rabbit anti-mouse brain serum.小鼠骨髓和脾源性多能造血干细胞（CFU-S）的免疫学比较：兔抗小鼠脑血清的作用
Biomedicine. 1978 Oct;29(6):191-3.
4
[Ultrastructural characteristics of bone marrow subendosteal region cells in T-cell virus-induced leukemia].[T细胞病毒诱导白血病中骨髓内皮下区域细胞的超微结构特征]
Eksp Onkol. 1984;6(3):24-9.
5
Suppressive effect of rabbit anti-mouse brain serum (RAMBS) on murine pluripotent hemopoietic precursors in vitro and committed early erythrocytic and macrophage-granulocytic precursors detected after brief in vivo passage.兔抗鼠脑血清（RAMBS）对体外培养的小鼠多能造血前体细胞以及经短暂体内传代后检测到的定向早期红细胞和巨噬细胞-粒细胞前体细胞的抑制作用。
Exp Hematol. 1982 Feb;10(2):228-33.
6
Surface antigens of murine hemopoietic stem cells. I. Cross reactivity of antisera against differentiated hemopoietic cells with bone marrow stem cells.小鼠造血干细胞的表面抗原。I. 抗分化造血细胞血清与骨髓干细胞的交叉反应性。
Exp Hematol. 1979 Oct;7(9):452-68.
7
[Ultrastructure of bone marrow mononuclear cells labeled with anti-stem cell serum by the PAP method].[采用PAP法用抗干细胞血清标记的骨髓单个核细胞的超微结构]
Gematol Transfuziol. 1983 Feb;28(2):38-42.
8
[Effect of anti-mouse brain serum on the CFUc in actively proliferating bone marrow].[抗小鼠脑血清对活跃增殖骨髓中集落形成单位（CFUc）的影响]
Radiobiologiia. 1986 Jul-Aug;26(4):553-7.
9
Direct identification of the murine pluripotential stem cell using rabbit anti-mouse brain serum.使用兔抗小鼠脑血清直接鉴定小鼠多能干细胞。
Transplantation. 1978 Jun;25(6):305-8. doi: 10.1097/00007890-197806000-00005.
10
Rabbit anti-mouse brain serum (RAMBS): lack of specific inhibition of late committed erythroid stem cells in culture (CFU-E).兔抗小鼠脑血清（RAMBS）：在培养中对晚期定向红细胞干细胞（CFU-E）缺乏特异性抑制作用。
Blut. 1978 Oct 13;37(4):211-4. doi: 10.1007/BF00996722.

本文引用的文献

1
A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.正常小鼠骨髓细胞辐射敏感性的直接测量。
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2
Suppressive effect of rabbit anti-mouse brain serum (RAMBS) on murine pluripotent hemopoietic precursors in vitro and committed early erythrocytic and macrophage-granulocytic precursors detected after brief in vivo passage.兔抗鼠脑血清（RAMBS）对体外培养的小鼠多能造血前体细胞以及经短暂体内传代后检测到的定向早期红细胞和巨噬细胞-粒细胞前体细胞的抑制作用。
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3
Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. I. Isolation of pluripotent hemopoietic stem cells and granulocyte-macrophage progenitor cells.
利用荧光激活细胞分选仪分析大鼠造血细胞。I. 多能造血干细胞和粒细胞-巨噬细胞祖细胞的分离。
J Exp Med. 1980 Aug 1;152(2):419-37. doi: 10.1084/jem.152.2.419.
4
Expression of Thy-1 antigen is not limited to T cells in cultures of mouse hemopoietic cells.在小鼠造血细胞培养物中，Thy-1抗原的表达并不局限于T细胞。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4161-5. doi: 10.1073/pnas.79.13.4161.
5
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.注入的辣根过氧化物酶在小鼠肾近端小管吸收的早期阶段：一种新技术的超微结构细胞化学研究
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6
Brain-associated stem cell antigen: an antigen shared by brain and hemopoietic stem cells.脑相关干细胞抗原：一种脑和造血干细胞共有的抗原。
J Exp Med. 1972 Aug 1;136(2):369-74. doi: 10.1084/jem.136.2.369.
7
[Possibility of detecting hematopoietic stem cells by electron microscopy].[通过电子显微镜检测造血干细胞的可能性]
Dokl Akad Nauk SSSR. 1974;217(1):207-8.
8
Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells.脑相关θ抗原：兔抗小鼠脑与小鼠淋巴细胞的反应性。
Cell Immunol. 1971 Aug;2(4):353-61. doi: 10.1016/0008-8749(71)90070-0.
9
Identification of cells in primate bone marrow resembling the hemopoietic stem cell in the mouse.灵长类动物骨髓中类似小鼠造血干细胞的细胞鉴定。
Blood. 1973 Aug;42(2):195-208.
10
Antigenic differences between hemopoietic stem cells and myeloid progenitors.造血干细胞与髓系祖细胞之间的抗原差异。
J Exp Med. 1974 Jun 1;139(6):1621-7. doi: 10.1084/jem.139.6.1621.