Van den Engh G J, Golub E S
J Exp Med. 1974 Jun 1;139(6):1621-7. doi: 10.1084/jem.139.6.1621.
Bone marrow contains pluripotent stem cells which give rise to colonies when injected into irradiated syngenic hosts as well as more differentiated progenitor cells of the myeloid cell which are able to form colonies in vitro. Antisera against brain is known to contain antistem cell antibody. The present experiments were designed to determine if the myeloid progenitor cell still expresses the stem cell antigen. Bone marrow cells were treated with antibrain antiserum plus complement and then survival of stem cells was determined by injection into irradiated hosts. Survival of myeloid progenitor cells was determined by culturing the cells in vitro. It was found that stem cells were eliminated by the antiserum but that myeloid progenitors were not. Inefficient in vitro lysis was ruled out as the reason for this difference since in vitro colonies were not reduced when the cells were treated with anti-immunoglobulin or after passage through an irradiated host. In the differentiation from stem cell to myeloid progenitor there is an associated surface antigen change.
骨髓含有多能干细胞，当将其注入经辐射的同基因宿主时可形成集落，骨髓中还含有更多分化的髓系祖细胞，这些祖细胞能够在体外形成集落。已知抗脑抗血清含有抗干细胞抗体。本实验旨在确定髓系祖细胞是否仍表达干细胞抗原。用抗脑抗血清加补体处理骨髓细胞，然后通过注入经辐射的宿主来测定干细胞的存活率。通过体外培养细胞来测定髓系祖细胞的存活率。发现抗血清可消除干细胞，但不能消除髓系祖细胞。体外裂解效率低下被排除为造成这种差异的原因，因为用抗免疫球蛋白处理细胞或使其通过经辐射的宿主后，体外集落并未减少。在从干细胞向髓系祖细胞的分化过程中，存在相关的表面抗原变化。
