用蛋白A和分离的兔肝细胞膜检测慢性肝病患者血清中的肝细胞膜抗体。
Liver cell membrane antibody detected by protein A and isolated rabbit liver plasma membrane in sera of patients with chronic liver diseases.
作者信息
Toda G, Ikeda Y, Hashimoto N, Yamazaki M, Torii M, Oka H
出版信息
Clin Exp Immunol. 1983 Dec;54(3):661-70.
Using the plasma membrane fraction isolated from rabbit liver (RLPM), we detected non-species specific IgG antibody against liver cell surface membrane in the sera from the patients with chronic liver diseases. The sea were treated with dithiothreitol and iodoacetamide, and absorbed with sufficient amount of actin isolated from rabbit striated muscles. The antibody was detected by incubation of RLPM with the treated and absorbed sera and subsequent determination of IgG bound to RLPM by 125I-staphylococcal protein A. It was found mainly in the patients with autoimmune hepatitis (12 of 28) and liver cirrhosis (eight of 24). It occurred more frequently in HBsAg negative liver cirrhosis than in HBsAg positive forms (six of 13 vs two of 11). The frequency of the antibody was low in chronic hepatitis except autoimmune hepatitis, and primary biliary cirrhosis. Thus the antibody against RLPM was an immunological marker of autoimmune hepatitis and HBsAg negative liver cirrhosis. The occurrence did not correlate with those of anti-smooth muscle antibody, anti-nuclear antibody and anti-mitochondrial antibody. In two cases of autoimmune hepatitis, the antibody against RLPM decreased with clinical improvement induced by corticosteroids.
利用从兔肝中分离得到的质膜组分（RLPM），我们在慢性肝病患者的血清中检测到了针对肝细胞表面膜的非种属特异性IgG抗体。血清经二硫苏糖醇和碘乙酰胺处理，并用从兔横纹肌中分离得到的足量肌动蛋白进行吸附。通过将RLPM与处理并吸附后的血清孵育，随后用125I - 葡萄球菌蛋白A测定与RLPM结合的IgG来检测该抗体。结果发现该抗体主要存在于自身免疫性肝炎患者（28例中有12例）和肝硬化患者（24例中有8例）中。在HBsAg阴性肝硬化患者中出现的频率高于HBsAg阳性肝硬化患者（13例中有6例 vs 11例中有2例）。除自身免疫性肝炎和原发性胆汁性肝硬化外，该抗体在慢性肝炎中的出现频率较低。因此，抗RLPM抗体是自身免疫性肝炎和HBsAg阴性肝硬化的一种免疫标志物。其出现与抗平滑肌抗体、抗核抗体和抗线粒体抗体的出现无关。在2例自身免疫性肝炎患者中，抗RLPM抗体随着皮质类固醇诱导的临床症状改善而降低。
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