Hussain Z, Bürger H, Lannigan R, Groves D J
J Clin Microbiol. 1984 Feb;19(2):215-7. doi: 10.1128/jcm.19.2.215-217.1984.
We investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. The biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. The standard reactions were compared with those obtained by preparing similar media, modified by containing no cysteine or resazurin, under aerobic conditions. Preincubation of the modified biochemical media with a standard suspension of a culture of Acinetobacter lwoffi provided reducing conditions. In 1,449 individual tests using 22 different biochemicals, 1,349 (93%) of the reactions agreed. Only two misidentifications resulted when using the modified media, one each at the species and genus levels. Two strains of Actinomyces odontolyticus failed to grow in coculture.
我们研究了一种通过与非发酵菌共培养来方便地降低氧张力并产生厌氧条件的方法。根据标准方法，使用预还原培养基和气相色谱法测定了代表41个不同物种的94株菌株的生化反应。将标准反应与在有氧条件下制备的不含半胱氨酸或刃天青的类似培养基所获得的反应进行比较。用洛菲不动杆菌培养物的标准悬液对改良生化培养基进行预孵育可提供还原条件。在使用22种不同生化物质的1449次单独测试中，1349次（93%）反应一致。使用改良培养基时仅出现两例假鉴定，分别在种和属水平各有一例。两株溶牙放线菌在共培养中未能生长。
