Whaley D N, Wiggs L S, Miller P H, Srivastava P U, Miller J M
Nosocomial Pathogens Laboratory Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1995 May;33(5):1196-202. doi: 10.1128/jcm.33.5.1196-1202.1995.
Identification of anaerobic bacteria requires special media and growth conditions that contribute to a higher cost per identification than that for aerobic isolates. Newer rapid methods streamline the identification process, but confirmation to the species level is often difficult. The Presumpto Plate method for the identification of commonly encountered anaerobes consists of three quadrant plates, each containing four conventional media, that result in the generation of 21 test parameters: growth on Lombard-Dowell medium; production of indole, indole derivative, catalase, lecithinase, and lipase; proteolysis of milk, H2S, and esculin; growth on 20% bile; precipitate on bile; DNase, glucose, casein, starch, and gelatin hydrolysis; and fermentation of lactose, mannitol, and rhamnose. Identification charts were developed by using the results from 2,300 anaerobic isolates. Because conventional media were used, there was a high degree of agreement between the Presumpto Plate method and the reference method when testing commonly encountered anaerobes. The Presumpto Plate method is as accurate as commercially available enzyme systems for the identification of many anaerobic species but is less expensive to perform.
厌氧菌的鉴定需要特殊的培养基和生长条件，这使得每次鉴定的成本高于需氧菌分离株。更新的快速方法简化了鉴定过程，但通常很难确定到种的水平。用于鉴定常见厌氧菌的预推定平板法由三个象限平板组成，每个平板包含四种传统培养基，可产生21个测试参数：在Lombard-Dowell培养基上生长；吲哚、吲哚衍生物、过氧化氢酶、卵磷脂酶和脂肪酶的产生；牛奶的蛋白水解、硫化氢和七叶苷；在20%胆汁上生长；胆汁沉淀；DNA酶、葡萄糖、酪蛋白、淀粉和明胶水解；以及乳糖、甘露醇和鼠李糖发酵。利用2300株厌氧菌分离株的结果绘制了鉴定图表。由于使用了传统培养基，在检测常见厌氧菌时，预推定平板法与参考方法之间具有高度一致性。预推定平板法在鉴定许多厌氧菌种类时与市售酶系统一样准确，但操作成本更低。
