Hussain Z, Lannigan R, Bürger H, Groves D
J Clin Microbiol. 1985 Apr;21(4):645-6. doi: 10.1128/jcm.21.4.645-646.1985.
A method to produce anaerobic conditions by coculture with a nonfermentative organism was utilized in conjunction with the Minitek anaerobe system (BBL Microbiology Systems, Cockeysville, Md.) for identification of anaerobic bacteria from clinical specimens. With the coculture method, the Minitek anaerobe identification tests could be incubated under aerobic conditions. In 1,900 individual biochemical reactions, 1,826 (96%) were identical whether anaerobic conditions were achieved by conventional or coculture techniques. In comparison with the reference identification (Virginia Polytechnic Institute and State University, Blacksburg), both systems of incubation identified 91 of 99 strains (92%) correctly. The method of incubation had an effect on identification to the genus level in 1 of 99 (1%) strains and to the species level in 3 of 99 (3%) strains.
一种通过与非发酵菌共培养来产生厌氧条件的方法，与Minitek厌氧菌系统（BBL微生物系统公司，马里兰州科基斯维尔）联合使用，用于从临床标本中鉴定厌氧菌。采用共培养方法时，Minitek厌氧菌鉴定试验可在有氧条件下进行培养。在1900次单独的生化反应中，无论通过传统技术还是共培养技术实现厌氧条件，1826次（96%）反应结果相同。与参考鉴定结果（弗吉尼亚理工学院和州立大学，布莱克斯堡）相比，两种培养系统对99株菌株中的91株（92%）鉴定正确。培养方法对99株中的1株（1%）菌株在属水平的鉴定以及99株中的3株（3%）菌株在种水平的鉴定有影响。
