金黄色葡萄球菌奥丁 - 豪肯斯抗原a5的免疫化学研究：一种含磷多糖。

Immunochemical studies of Staphylococcus aureus Oeding-Haukenes antigen a5: a phosphorus-containing polysaccharide.

作者信息

Ndulue A N, Flandrois J P

出版信息

J Gen Microbiol. 1983 Dec;129(12):3603-10. doi: 10.1099/00221287-129-12-3603.

Abstract

Antigen a5 was isolated from strain 830 of Staphylococcus aureus by autolysis in phosphate buffer followed by alcohol precipitation. Purification was principally achieved by affinity chromatography on wheat germ agglutinin ultrogel and on anti-S. aureus teichoic acid immunosorbent. The a5 antigen was weakly immunogenic in rabbits. Chemical analysis showed that a5 is a teichoic acid composed of ribitol phosphate, N-acetylglucosamine and alanine. It has similar physico-chemical properties to the wall beta-N-acetylglucosamine ribitol teichoic acid of S. aureus but is serologically distinct.

摘要

通过在磷酸盐缓冲液中自溶，随后进行乙醇沉淀，从金黄色葡萄球菌830菌株中分离出抗原a5。主要通过在麦胚凝集素琼脂糖凝胶和抗金黄色葡萄球菌磷壁酸免疫吸附剂上进行亲和层析来实现纯化。a5抗原在兔体内免疫原性较弱。化学分析表明，a5是一种由磷酸核糖醇、N-乙酰葡糖胺和丙氨酸组成的磷壁酸。它具有与金黄色葡萄球菌的细胞壁β-N-乙酰葡糖胺核糖醇磷壁酸相似的物理化学性质，但在血清学上是不同的。