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用于检测抗金黄色葡萄球菌核糖醇磷壁酸抗体的乳胶试剂的制备。

Preparation of a latex reagent for the detection of anti-Staphylococcus aureus ribitol teichoic acid antibodies.

作者信息

de Montclos M, Flandrois J P

机构信息

Bacteriology Laboratory, Université Claude Bernard Lyon I, Faculté de Médecine Lyon-Sud, Pierre-Benite, France.

出版信息

Zentralbl Bakteriol. 1990 Oct;274(1):50-60. doi: 10.1016/s0934-8840(11)80974-6.

DOI:10.1016/s0934-8840(11)80974-6
PMID:2261065
Abstract

Purified S. aureus ribitol teichoic acid was covalently bound to carboxylated latex particles. The immunological properties of the polysaccharide antigen were preserved. The reagent obtained was used for the quantification of anti-ribitol teichoic acid antibodies by means of a direct and rapid agglutination test carried out on a slide. There was good correlation between the preliminary results of this test and those obtained with counter-immunoelectrophoresis (CIE). The method is faster and more sensitive than CIE.

摘要

纯化的金黄色葡萄球菌核糖醇磷壁酸与羧化乳胶颗粒共价结合。多糖抗原的免疫特性得以保留。所获得的试剂用于通过在载玻片上进行的直接快速凝集试验来定量抗核糖醇磷壁酸抗体。该试验的初步结果与免疫对流电泳(CIE)获得的结果之间具有良好的相关性。该方法比CIE更快且更灵敏。

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