Morimoto S, Matsumura Y, Ohno Y, Miyawaki N
J Pharmacobiodyn. 1983 Nov;6(11):844-50. doi: 10.1248/bpb1978.6.844.
The present study was carried out to investigate the effects of vitamin E-deficiency and supplementation of alpha-tocopheryl acetate (TOCA) on renin release from renin granules. Male Wistar rats were fed either a control or a vitamin E-deficient diet for 4 weeks. Subsequently, the vitamin E-deficient rats received dietary supplementation of TOCA (40 mg/100 g diet) for 5 d. The renin granule fraction was prepared from the kidney cortex homogenate by a discontinuous sucrose density gradient centrifugation. The intake of vitamin E-deficient diet for 4 weeks resulted in an increased level of endogenous lipid peroxides in the renin granule fraction, accompanied by a marked decrease in alpha-tocopherol content, and led to a significant increase in the rate of renin release from the granules during incubation at 37 degrees C. These changes in alpha-tocopherol content, lipid peroxide level and renin release in the renin granule fraction were restored to the control values by dietary TOCA supplementation. Similarly, dietary supplementation of N,N'-diphenyl-p-phenylenediamine (80 mg/100 g diet), which has an antioxidative ability, suppressed the increases in lipid peroxidation and renin release due to vitamin E-deficiency, although this compound was ineffective in restoring alpha-tocopherol levels. These results suggest that vitamin E functions in maintenance of membrane integrity of renin granules by inhibiting the lipid peroxidation.
本研究旨在探讨维生素E缺乏及补充醋酸生育酚（TOCA）对肾素颗粒释放肾素的影响。将雄性Wistar大鼠分别喂以对照饮食或维生素E缺乏饮食4周。随后，给维生素E缺乏的大鼠在饮食中补充TOCA（40毫克/100克饮食），持续5天。通过不连续蔗糖密度梯度离心从肾皮质匀浆中制备肾素颗粒部分。4周的维生素E缺乏饮食摄入导致肾素颗粒部分内源性脂质过氧化物水平升高，同时生育酚含量显著降低，并导致在37℃孵育期间颗粒释放肾素的速率显著增加。通过饮食补充TOCA，肾素颗粒部分中生育酚含量、脂质过氧化物水平和肾素释放的这些变化恢复到对照值。同样，具有抗氧化能力的N，N'-二苯基对苯二胺（80毫克/100克饮食）的饮食补充抑制了由于维生素E缺乏引起的脂质过氧化和肾素释放的增加，尽管该化合物在恢复生育酚水平方面无效。这些结果表明，维生素E通过抑制脂质过氧化作用来维持肾素颗粒的膜完整性。
