Morimoto S, Miyawaki N, Matsumura Y, Ohno Y, Mizunoya Y
J Pharmacobiodyn. 1983 Nov;6(11):892-5. doi: 10.1248/bpb1978.6.892.
Effect of vitamin E-deficiency on renin release was examined with rat kidney cortical slices. Male Wistar rats were fed either a control or a vitamin E-deficient diet for 4 weeks. When kidney cortical slices were incubated in a Krebs-Ringers' bicarbonate solution (pH 7.4) at 37 degrees C, the rate of renin release into the incubation medium in vitamin E-deficient group was significantly higher than that in the control group. However, dietary supplementation of alpha-tocopheryl acetate (TOCA) or N,N'-diphenyl-p-phenylenediamine (DPPD) to the vitamin E-deficient rats for 5 d suppressed the stimulation of renin release from kidney cortical slices by vitamin E-deficiency. On the other hand, the release of protein, acid phosphatase and alkaline phosphatase during incubation of kidney cortical slices was not affected by vitamin E-deficiency or supplementations of TOCA and DPPD. These findings indicate that vitamin E-deficiency specifically stimulates renin release from kidney cortical slices and this effect is attenuated by the dietary supplementation of TOCA or DPPD.
采用大鼠肾皮质切片研究维生素E缺乏对肾素释放的影响。将雄性Wistar大鼠分别给予对照饮食或维生素E缺乏饮食4周。当肾皮质切片在37℃的 Krebs-Ringer碳酸氢盐溶液（pH 7.4）中孵育时，维生素E缺乏组肾素释放到孵育培养基中的速率显著高于对照组。然而，给维生素E缺乏的大鼠饮食补充醋酸α-生育酚（TOCA）或N，N'-二苯基对苯二胺（DPPD）5天，可抑制维生素E缺乏对肾皮质切片肾素释放的刺激作用。另一方面，肾皮质切片孵育期间蛋白质、酸性磷酸酶和碱性磷酸酶的释放不受维生素E缺乏或TOCA和DPPD补充的影响。这些发现表明，维生素E缺乏特异性刺激肾皮质切片释放肾素，而饮食补充TOCA或DPPD可减弱这种作用。
