在未切片、扁平的青蛙视顶盖中可视化辣根过氧化物酶填充的轴突。
Visualization of HRP-filled axons in unsectioned, flattened optic tecta of frogs.
作者信息
Udin S B, Fisher M D
出版信息
J Neurosci Methods. 1983 Dec;9(4):283-5. doi: 10.1016/0165-0270(83)90059-6.
PMID:6366381
Abstract
In order to trace individual axons in the tectum, a curved structure, we have modified the HRP method of Adams for use on unsectioned, flattened tecta. Filled axons appear dark and uniformly filled and can be followed without the necessity for reconstructions from serial sections.
摘要
为了追踪中脑顶盖（一种弯曲结构）中的单个轴突，我们对亚当斯的辣根过氧化物酶（HRP）方法进行了改进，以便用于未切片、展平的中脑顶盖。填充后的轴突呈现深色且均匀填充，无需从连续切片进行重建即可追踪。
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