Sciaky D, Thomashow M F
Nucleic Acids Res. 1984 Feb 10;12(3):1447-61. doi: 10.1093/nar/12.3.1447.
The incorporation of Ti plasmid sequences, the T-DNA, into the genomes of dicotyledenous plants causes the formation of tumors. Here we report the nucleotide sequence of one of the T-DNA "oncogenes", the transcript 2 gene of pTiA6 and we further characterize the 2.7 Kb element that has spontaneously inserted into this gene in plasmid pTiA66. The results indicate that the transcript 2 portion of the T-DNA has an open reading frame that could encode a polypeptide of 49.8 Kd. The open reading frame is surrounded by sequences that typically have roles in eucaryotic gene expression. Nucleotide sequence and Southern blot analysis also indicates that the 2.7 Kb insert in the transcript 2 gene of pTiA66 is located within the coding sequence of the gene and suggests that the element is an insertion sequence. We designate this element, IS66.
Ti质粒序列（T-DNA）整合到双子叶植物基因组中会导致肿瘤形成。在此我们报告T-DNA“致癌基因”之一——pTiA6转录本2基因的核苷酸序列，并进一步鉴定在质粒pTiA66中自发插入该基因的2.7 Kb元件。结果表明，T-DNA的转录本2部分有一个开放阅读框，可编码一个49.8 Kd的多肽。该开放阅读框被通常在真核基因表达中起作用的序列所环绕。核苷酸序列和Southern印迹分析还表明，pTiA66转录本2基因中的2.7 Kb插入片段位于该基因的编码序列内，提示该元件是一个插入序列。我们将此元件命名为IS66。
