Johnson L L, Dove W F
Immunogenetics. 1984;19(3):233-41. doi: 10.1007/BF00364766.
Male-specific antigen expression by XY teratocarcinomas PCC7 and 7' is demonstrated first by the rejection of tumors by female but not by male mice following challenge with these cell lines. Male-specific antigen expression is confirmed by an indirect method in which females are immunized against H-Y antigen by male skin grafts. A variant of PCC7 lacking male-specific antigen expression is described. Analysis of the karyotype and of the DNA from this variant indicate that the loss of male-specific antigen expression is a result of the loss of the Y chromosome. The ability to recover variants that have lost expression of male-specific antigen opens the possibility of their selection after mutagenesis.
XY 畸胎瘤 PCC7 和 7' 的雄性特异性抗原表达首先通过用这些细胞系攻击后雌性小鼠而非雄性小鼠排斥肿瘤得以证明。雄性特异性抗原表达通过一种间接方法得以证实，即通过雄性皮肤移植使雌性小鼠针对 H-Y 抗原进行免疫。描述了一种缺乏雄性特异性抗原表达的 PCC7 变体。对该变体的核型和 DNA 分析表明，雄性特异性抗原表达的丧失是 Y 染色体丢失的结果。恢复已丧失雄性特异性抗原表达的变体的能力为诱变后对其进行选择提供了可能性。
