Graves P M, Carter R, Keystone J S, Seeley D C
Am J Trop Med Hyg. 1984 Mar;33(2):212-9. doi: 10.4269/ajtmh.1984.33.212.
Clones have been derived by limiting dilution from recent isolates of Plasmodium falciparum from Africa and Brazil. These clones have been characterized for pyrimethamine and chloroquine sensitivity and/or isoenzyme type. Clones obtained from an isolate containing a mixture of GPI isoenzyme types were found to have (with one exception) only GPI-1 or GPI-2. Clones differing 1,000-fold in pyrimethamine resistance were derived from a single isolate from Brazil which was resistant to both pyrimethamine and chloroquine. Several levels of chloroquine sensitivity were observed in the isolates studied. All clones showed levels of chloroquine sensitivity similar to that of their parent isolates. The KZ1 isolate from East Africa had an intermediate level of chloroquine resistance and a flat dose-response curve and each of its clones had a similar dose-response curve, indicating that the intermediate level of chloroquine resistance did not result from the KZ1 isolate's being a mixture of clones with high and low sensitivity.
已通过有限稀释法从非洲和巴西近期分离出的恶性疟原虫中获得克隆。这些克隆已针对乙胺嘧啶和氯喹敏感性及/或同工酶类型进行了表征。从含有多种糖基磷脂酰肌醇（GPI）同工酶类型混合物的分离物中获得的克隆（有一个例外）被发现仅具有GPI - 1或GPI - 2。从巴西的一个对乙胺嘧啶和氯喹均耐药的单一分离物中获得了对乙胺嘧啶耐药性相差1000倍的克隆。在所研究的分离物中观察到了几种氯喹敏感性水平。所有克隆显示出的氯喹敏感性水平与其亲本分离物相似。来自东非的KZ1分离物具有中等水平的氯喹耐药性和一条平坦的剂量反应曲线，其每个克隆都有类似的剂量反应曲线，这表明氯喹的中等耐药水平并非源于KZ1分离物是高敏感性和低敏感性克隆的混合物。
