Detection of canine antisperm antibodies by indirect immunofluorescence and gelatin agglutination.

Canine infertility has diverse and multiple causes. Immunologic infertility due to antisperm antibody is recognized and quantitated in other species, but this entity appears not to have been described in the dog. Before this time, there has been no practicable and reliable method to measure canine antisperm antibodies. This study was directed at inducing the production of such antibodies by immunizing dogs with sperm antigens and measuring the resulting antibodies, using the recognized techniques of indirect fluorescent antibody testing and sperm agglutination testing. Measurable antibody responses were observed.