微小菌素D93对大肠杆菌菌株的分离、特性鉴定及作用模式
Isolation, characterization, and mode of action on Escherichia coli strains of microcin D93.
作者信息
Martínez J L, Pérez-Díaz J C
出版信息
Antimicrob Agents Chemother. 1986 Mar;29(3):456-60. doi: 10.1128/AAC.29.3.456.
Abstract
Microcin D93 is an antibiotic substance produced by Escherichia coli strains which harbor the 5.5-kilobase plasmid pMccD93. Its production is unaffected by the use of different carbon and ammonia sources, different phosphate concentrations, or mitomycin C. We developed a method for purifying this microcin based on gel permeation chromatography and reverse-phase high-pressure liquid chromatography. The antibiotic appears to be a small, hydrophilic, basic peptide, active on E. coli and Proteus, Citrobacter, and Pseudomonas species and much more active on recA strains than on their isogenic wild type. Diminution of the rate of DNA biosynthesis without any apparent effect on other macromolecules appears to be a primary effect in the action of microcin D93.
摘要
微菌素D93是由携带5.5千碱基质粒pMccD93的大肠杆菌菌株产生的一种抗生素物质。其产生不受不同碳源和氨源、不同磷酸盐浓度或丝裂霉素C的使用影响。我们开发了一种基于凝胶渗透色谱和反相高压液相色谱纯化这种微菌素的方法。这种抗生素似乎是一种小的、亲水性的碱性肽，对大肠杆菌、变形杆菌、柠檬酸杆菌和假单胞菌属有活性，并且对recA菌株的活性比对其同基因野生型的活性高得多。在微菌素D93的作用中，DNA生物合成速率的降低而对其他大分子没有明显影响似乎是主要作用。
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本文引用的文献
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