Salomón R A, Farías R N
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas-UNT), Tucumán, Argentina.
J Bacteriol. 1992 Nov;174(22):7428-35. doi: 10.1128/jb.174.22.7428-7435.1992.
Microcin 25, a peptide antibiotic excreted by an Escherichia coli strain isolated from human feces, was purified to homogeneity and characterized. Composition analysis and data from gel filtration indicated that microcin 25 may contain 20 amino acid residues. It has a blocked amino-terminal end. Microcin synthesis and immunity are plasmid determined, and the antibiotic was produced in minimal medium when the cultures entered the stationary phase of growth. The peptide appears to interfere with cell division, since susceptible cells filamented when exposed to it. This response does not seem to be mediated by the SOS system.
微菌素25是一种从人类粪便中分离出的大肠杆菌菌株分泌的肽抗生素，已被纯化至同质并进行了特性鉴定。组成分析和凝胶过滤数据表明，微菌素25可能含有20个氨基酸残基。它的氨基末端是封闭的。微菌素的合成和免疫由质粒决定，当培养物进入生长稳定期时，该抗生素在基本培养基中产生。该肽似乎会干扰细胞分裂，因为敏感细胞在接触它时会形成丝状体。这种反应似乎不是由SOS系统介导的。
