大肠杆菌产生的抗菌肽微菌素7的结构与作用模式
Structure and mode of action of microcin 7, an antibacterial peptide produced by Escherichia coli.
作者信息
Garcia-Bustos J F, Pezzi N, Mendez E
出版信息
Antimicrob Agents Chemother. 1985 May;27(5):791-7. doi: 10.1128/AAC.27.5.791.
Abstract
Microcin 7 is a small peptide produced and excreted to the culture medium by stationary-phase Escherichia coli cells harboring the pMccC7 plasmid (formerly named pRYC7). This peptide inhibited the growth of the enterobacteria phylogenetically closer to E. coli, apparently by blocking protein biosynthesis. The molecule was degraded with trypsin, and the resulting fragments were purified and sequenced. The results show that microcin 7 is a linear heptapeptide blocked at both ends.
摘要
微菌素7是一种由携带pMccC7质粒（原名为pRYC7）的稳定期大肠杆菌细胞产生并分泌到培养基中的小肽。这种肽明显通过阻断蛋白质生物合成来抑制与大肠杆菌系统发育关系更近的肠杆菌的生长。该分子用胰蛋白酶降解，所得片段经纯化和测序。结果表明，微菌素7是一种两端封闭的线性七肽。
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本文引用的文献
1
THE AMINO ACID SEQUENCE OF THE GAMMA CHAIN OF HUMAN FETAL HEMOGLOBIN.人类胎儿血红蛋白γ链的氨基酸序列
Biochemistry. 1963 Sep-Oct;2:992-1008. doi: 10.1021/bi00905a016.
2
The amino acid sequence around the reactive serine residue of some proteolytic enzymes.一些蛋白水解酶的活性丝氨酸残基周围的氨基酸序列。
Biochem J. 1960 Oct;77(1):149-63. doi: 10.1042/bj0770149.
3
The amino acid sequence of the delta haemolysin of Staphylococcus aureus.金黄色葡萄球菌δ溶血素的氨基酸序列。
FEBS Lett. 1980 Jun 30;115(2):209-12. doi: 10.1016/0014-5793(80)81170-7.
4
The mechanism of protein secretion across membranes.蛋白质跨膜分泌的机制。
Nature. 1980 Jan 31;283(5746):433-8. doi: 10.1038/283433a0.
5
Physical characterization of plasmids determining synthesis of a microcin which inhibits methionine synthesis in Escherichia coli.决定合成一种抑制大肠杆菌蛋氨酸合成的微菌素的质粒的物理特性
J Bacteriol. 1980 Mar;141(3):1015-23. doi: 10.1128/jb.141.3.1015-1023.1980.
6
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7
Primary structure of corticotropin-releasing factor from ovine hypothalamus.来自绵羊下丘脑的促肾上腺皮质激素释放因子的一级结构。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6517-21. doi: 10.1073/pnas.78.10.6517.
8
Conferral of transposable properties to a chromosomal gene in Escherichia coli.赋予大肠杆菌中一个染色体基因转座特性
J Mol Biol. 1980 Aug 15;141(3):235-48. doi: 10.1016/0022-2836(80)90179-5.
9
A protein sequenator.蛋白质测序仪。
Eur J Biochem. 1967 Mar;1(1):80-91. doi: 10.1007/978-3-662-25813-2_14.
10
Structure of alpha-1-CB8, a large cyanogen bromide produced fragment from the alpha-1 chain of rat collagen. The nature of a hydroxylamine-sensitive bond and composition of tryptic peptides.α-1-CB8的结构，一种由大鼠胶原蛋白α-1链经溴化氰裂解产生的大片段。一种对羟胺敏感的键的性质及胰蛋白酶肽段的组成。
Biochemistry. 1970 Jun 9;9(12):2408-21. doi: 10.1021/bi00814a004.
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