高效液相色谱-化学发光检测法测定人血清中的肌红蛋白

Determination of myoglobin in human serum by high-performance liquid chromatography with chemiluminescence detection.

作者信息

Maltsev V G, Zimina T M, Khvatov A B, Belenkii B G

出版信息

J Chromatogr. 1987 Apr 24;416(1):45-52. doi: 10.1016/0378-4347(87)80483-8.

Abstract

A highly sensitive method for the determination of myoglobin in serum is described, based on high-performance size-exclusion chromatography with chemiluminescence detection. Serum proteins are separated according to their molecular masses on columns packed with TSK-SW gel and those containing haem are detected selectively by post-column chemiluminescence reaction with luminol using a conventional fluorimetric detector. The method is rapid (30 min) and sufficiently sensitive for the diagnosis of myocardial infarction. The minimum detectable myoglobin concentration is 10 ng/ml.

摘要

本文描述了一种基于高效排阻色谱-化学发光检测法测定血清中肌红蛋白的高灵敏度方法。血清蛋白在填充有TSK-SW凝胶的柱上根据其分子量进行分离，含血红素的蛋白通过使用传统荧光检测器与鲁米诺进行柱后化学发光反应来选择性检测。该方法快速（30分钟），对心肌梗死的诊断具有足够的灵敏度。肌红蛋白的最低检测浓度为10 ng/ml。