Triebling A T, Długosz J, Brzozowski J, Andrzejewska A, Wereszczyńska U, Gabryelewicz A
Pathol Res Pract. 1984 Jan;178(3):280-8. doi: 10.1016/S0344-0338(84)80111-9.
The inflammatory process in pancreas affects the function and structure of kidneys both by enzymatic toxemia and impairment of the renal circulation. In this study the stability of renal lysosomes in AEP in dogs treated with cytoprotective agent PGI2 was investigated. AEP was induced by injection of the bile and trypsin into the pancreatic duct; experiments were terminated after 12 hours. In lysosomal enriched subfraction of the kidney cortex (sedimenting in 15 000 x g) in untreated group (N = 5) relative free activity (r.f.a.) of cathepsins (Cs), acid phosphatase (APh) and beta-glucuronidase (BG) increased to 51,67 and 62% respectively, whereas in healthy dogs (N = 6) these activities were 20,38 and 25%. In dogs (N = 6) treated with PGI2 at the dose of 20 ng/kg/min. during 12 hrs, the r.f.a. of Cs, APh and BG was 18,40 and 49%, whereas in dogs (N = 5) additionally pretreated during 1 hr before induction of AEP with the same dose of PGI2, its values achieved 19,40 and 47% respectively. Our results suggest the stabilizing effect of PGI2 on kidney lysosomes damaged in acute experimental pancreatitis in dog. As possible mechanisms of prostacyclin action are discussed: limitation of necrotic process in the pancreas; improvement of renal haemodynamics; direct cytoprotective effect on the kidney.
胰腺中的炎症过程通过酶性毒血症和肾循环损伤影响肾脏的功能和结构。在本研究中，研究了用细胞保护剂前列环素（PGI2）治疗的犬急性实验性胰腺炎（AEP）中肾脏溶酶体的稳定性。通过将胆汁和胰蛋白酶注入胰管诱导AEP；12小时后终止实验。在未治疗组（N = 5）的肾皮质溶酶体富集亚组分（在15000×g下沉淀）中，组织蛋白酶（Cs）、酸性磷酸酶（APh）和β-葡萄糖醛酸酶（BG）的相对游离活性（r.f.a.）分别增加到51%、67%和62%，而在健康犬（N = 6）中这些活性分别为20%、38%和25%。在以20 ng/kg/min的剂量给予PGI2治疗12小时的犬（N = 6）中，Cs、APh和BG的r.f.a.分别为18%、40%和49%，而在诱导AEP前1小时用相同剂量的PGI2进行预处理的犬（N = 5）中，其值分别达到19%、40%和47%。我们的结果表明PGI2对犬急性实验性胰腺炎中受损的肾脏溶酶体具有稳定作用。文中讨论了前列环素作用的可能机制：限制胰腺中的坏死过程；改善肾脏血液动力学；对肾脏的直接细胞保护作用。
