Długosz J, Bajko K, Gabryelewicz A
Exp Pathol (Jena). 1979;17(5):237-42. doi: 10.1016/s0014-4908(79)80037-x.
In 12 dogs with acute experimental pancreatitis (AEP) and 6 control animals the "free", "latent" and "total" activity of acid phosphatase, beta-glucuronidase and cathepsins in whole homogenates of the pancreas, in a lysosomal-enriched subfraction and the supernatant of pancreatic tissue was estimated. AEP was induced by injection of bile salts and thrombin solution into the pancreatic duct. In 6 dogs the protection with heparin (1.5 mg/kg/body weight) immediately after producing AEP was applied. In AEP without any protection the free activity of hydrolases in the whole homogenate (80--90%) and in the lysosomal enriched subfraction (75--90%) was higher than in the controls (60--70% and 55--75% respectively), suggesting an augmented lysosomal fragility during the course of AEP. Heparin depressed the free activity of hydrolases to 60--80% in whole homogenates, and 64--75% in the lysosomal enriched subfraction. The release of cathepsins during incubation of the lysosomal-enriched subfraction in acidic medium was lower in the group with heparin treatment. The data obtained suggest the stabilising effect of heparin on the lysosomes of the pancreas during acute experimental pancreatitis in dogs.
在12只患有急性实验性胰腺炎（AEP）的犬和6只对照动物中，对胰腺全匀浆、富含溶酶体的亚组分以及胰腺组织上清液中酸性磷酸酶、β-葡萄糖醛酸酶和组织蛋白酶的“游离”、“潜在”和“总”活性进行了评估。通过向胰管注射胆盐和凝血酶溶液诱导AEP。在6只犬中，在产生AEP后立即给予肝素（1.5mg/kg体重）进行保护。在未给予任何保护的AEP中，全匀浆（80 - 90%）和富含溶酶体的亚组分（75 - 90%）中水解酶的游离活性高于对照组（分别为60 - 70%和55 - 75%），这表明在AEP过程中溶酶体脆性增加。肝素使全匀浆中水解酶的游离活性降低至60 - 80%，在富含溶酶体的亚组分中降低至64 - 75%。在酸性培养基中孵育富含溶酶体的亚组分时，肝素治疗组组织蛋白酶的释放较低。获得的数据表明，肝素对犬急性实验性胰腺炎期间胰腺的溶酶体具有稳定作用。
