Dlugosz J, Gabryelewicz A, Bajko K
Acta Hepatogastroenterol (Stuttg). 1977 Feb;24(1):44-51.
In dogs with acute experimental pancreatitis (AEP) induced according to Elliotts method the total, free and latent activity of lysosomal hydrolases (acid phosphatase, beta-glucuronidase and cathepsins) in whole homogenates and some subfractions of pancreas were studied. The animals were divided into three groups of 6 dogs each: I. control healthy dogs. II. AEP-treated with glucagon (0.33 mg of glucagon in drop infusion 3 times every six hours). III. AEP without any drug treatment. In dogs treated with glucagon the significant decrease of relative free activity of all tested hydrolases (66-80%) in comparison with the group without any treatment (III/80-90%) was found. Moreover significant decrease of total catheptic activity (about 1/3) in the former group was demonstrated. Incubation of lysosomal enriched fraction taken from group II/in medium buffered to pH 5.0 caused decreasing release of catheptic activity (60% of total) in comparison with the group III (75%). The histochemical reaction for acid phosphatase according to Gomoris method in pancreatic acinar cells of dogs treated with glucagon was less intensive than reaction in untreated animals. These results indicate on the less impairment of pancreatic lysosomes in AEP treated with glucagon in comparison with that in untreated animals.
在按照埃利奥特方法诱导产生急性实验性胰腺炎（AEP）的犬类中，研究了胰腺全匀浆及部分亚组分中溶酶体水解酶（酸性磷酸酶、β-葡萄糖醛酸酶和组织蛋白酶）的总活性、游离活性和潜在活性。动物被分为三组，每组6只犬：I. 对照健康犬。II. 用胰高血糖素治疗的AEP犬（每6小时静脉滴注0.33毫克胰高血糖素，共3次）。III. 未进行任何药物治疗的AEP犬。与未治疗组（III组，80 - 90%）相比，用胰高血糖素治疗的犬中，所有测试水解酶的相对游离活性显著降低（66 - 80%）。此外，前一组的总组织蛋白酶活性显著降低（约1/3）。与III组（75%）相比，将II组富含溶酶体的组分在pH 5.0的缓冲培养基中孵育，组织蛋白酶活性的释放减少（占总量的60%）。按照戈莫里方法对用胰高血糖素治疗的犬胰腺腺泡细胞进行酸性磷酸酶的组织化学反应，其强度低于未治疗动物的反应。这些结果表明，与未治疗的动物相比，用胰高血糖素治疗的AEP犬胰腺溶酶体的损伤较小。
