Długosz J W, Triebling A T, Brzozowski J
Department of Gastroenterology, Medical Academy, Białystok, Poland.
Mater Med Pol. 1993 Jul-Dec;25(3-4):119-25.
The role of lysosomal hydrolases in the pathogenesis of acute pancreatitis and secondary liver injury, as an important aspect of multisystem organ failure, remains unclear. The purpose of this study was to assess the lysosomal fragility in both organs in acute experimental pancreatitis (AEP) of graded severity in dogs. In 7 dogs, the moderate (M) and in 13 dogs severe (S) variant of bile--trypsin AEP--was induced; 6 dogs were in control group (C). The 24 h survival time was 6/7 and 6/13, respectively. After that time, the dogs were sacrificed and the lysosomal enriched subfraction (L) from both organs was isolated by ultracentrifugation. The total (T) and free (F) activities of beta-glucuronidase (beta G), cathepsins (Cs) and acid phosphatase (AcP) according to Gianetto and de Duve were assayed. The fractional free activity (% F/T) was adapted as and index of lysosomal stability. The %F/T of BG in the homogenate of the pancreas in AEP(S) was higher than that in AEP(M) (92% vs. 71%, p < 0.05, and vs. 37% in C, p < 0.005). The %F/T of Cs and AcP showed a similar pattern. The %F/T of beta G in L of the liver in AEP(S) was 38% vs. 29% in AEP(M), (p < 0.05), and vs. 20% in C (p < 0.05). In AEP in dogs the %F/T activities of lysosomal hydrolases in the pancreas and liver were increased, suggesting the labilization of lysosomal membranes in severe form of this disease. Our results support the pathogenic role of lysosomal hydrolases in the damage to the pancreas and liver in acute pancreatitis.
溶酶体水解酶在急性胰腺炎发病机制及继发性肝损伤（多系统器官功能衰竭的一个重要方面）中的作用仍不明确。本研究旨在评估犬分级严重性急性实验性胰腺炎（AEP）中两个器官的溶酶体脆性。对7只犬诱发中度（M）胆汁 - 胰蛋白酶AEP，对13只犬诱发重度（S）胆汁 - 胰蛋白酶AEP；6只犬作为对照组（C）。24小时存活时间分别为6/7和6/13。此后，处死犬并通过超速离心从两个器官中分离出富含溶酶体的亚组分（L）。根据Gianetto和de Duve的方法测定β - 葡萄糖醛酸酶（βG）、组织蛋白酶（Cs）和酸性磷酸酶（AcP）的总活性（T）和游离活性（F）。将游离活性分数（%F/T）用作溶酶体稳定性指标。AEP（S）组胰腺匀浆中BG的%F/T高于AEP（M）组（92%对71%，p < 0.05，与C组的37%相比，p < 0.005）。Cs和AcP的%F/T呈现类似模式。AEP（S）组肝脏L中βG的%F/T为38%，AEP（M）组为29%（p < 0.05），与C组的20%相比（p < 0.05）。在犬AEP中，胰腺和肝脏中溶酶体水解酶的%F/T活性增加，表明在该疾病的严重形式中溶酶体膜不稳定。我们的结果支持溶酶体水解酶在急性胰腺炎中对胰腺和肝脏损伤的致病作用。
