Hahn G L, Polgar P R
Atherosclerosis. 1984 Apr;51(1):143-50. doi: 10.1016/0021-9150(84)90150-3.
Bovine pulmonary artery endothelial cells, maintained in culture for prolonged periods, were observed to enter into a secondary growth pattern commonly referred to as sprouting. Our investigations, as well as those of other workers, indicate that these cells represent a phenotypic variant of the original endothelial cell population, rather than the proliferation of a contaminating cell type. A significant increase in prostaglandin production, particularly prostacyclin, could be correlated with the appearance of these phenotypically distinct endothelial cells in culture. We suggest that the elevation in prostacyclin synthesis is a result of endothelial cell sprouting and hypothesize that increased prostacyclin levels may account for the inhibition of platelet deposition reported in post-confluent endothelial cultures.
长时间培养的牛肺动脉内皮细胞被观察到进入一种通常称为发芽的二次生长模式。我们以及其他研究人员的调查表明，这些细胞代表原始内皮细胞群体的表型变体，而不是污染细胞类型的增殖。前列腺素产生，特别是前列环素的显著增加，可能与培养中这些表型不同的内皮细胞的出现有关。我们认为前列环素合成的升高是内皮细胞发芽的结果，并假设前列环素水平的增加可能解释汇合后内皮细胞培养物中报道的血小板沉积抑制现象。
