Araki A, Muramatsu M, Matsushima T
Gan. 1984 Jan;75(1):8-16.
The mutagenicities of twelve N-nitrosamines were tested on Salmonella typhimurium TA100 and Escherichia coli WP2 uvrA/pKM101 in the presence of rat liver S9 or hamster liver S9 by "preincubation" and "pour-plate" assays. The following eleven N-nitrosamines were mutagenic: N-nitroso-N-dimethylamine; N-nitroso-N-diethylamine; N-nitroso-N-di-n-propylamine; N-nitroso-N-di-n-butylamine; N-nitroso-N-methyl-n- amylamine ; N-nitroso-piperidine; N-nitrosomorpholine; N-nitroso-N-methyl-piperazine; N-nitroso-N-methyl-benzylamine; N-nitroso-N-methyl- phenylamine and N-nitroso-N-phenyl-benzylamine. N-Nitroso-N-diphenylamine was not mutagenic. E. coli WP2 uvrA/pKM101 was more sensitive than S. typhimurium TA100 to the mutagenic actions of N-nitroso-dialkyl amines, N-nitroso- aklyl -aryl amines and N-nitroso- diaryl amines, but S. typhimurium TA100 was more sensitive to those of N-nitroso-cyclic amines. Hamster liver S9 was better than rat liver S9 for metabolic activation of N-nitrosamines, and the preincubation step enhanced the mutagenicities of N-nitrosamines.
采用“预孵育”和“倾注平板”试验，在大鼠肝脏S9或仓鼠肝脏S9存在的情况下，对12种N-亚硝胺在鼠伤寒沙门氏菌TA100和大肠杆菌WP2 uvrA/pKM101上的致突变性进行了测试。以下11种N-亚硝胺具有致突变性：N-亚硝基-N-二甲胺；N-亚硝基-N-二乙胺；N-亚硝基-N-二正丙胺；N-亚硝基-N-二正丁胺；N-亚硝基-N-甲基-n-戊胺；N-亚硝基哌啶；N-亚硝基吗啉；N-亚硝基-N-甲基哌嗪；N-亚硝基-N-甲基苄胺；N-亚硝基-N-甲基苯胺和N-亚硝基-N-苯基苄胺。N-亚硝基-N-二苯胺没有致突变性。大肠杆菌WP2 uvrA/pKM101对N-亚硝基二烷基胺、N-亚硝基烷基芳基胺和N-亚硝基二芳基胺的致突变作用比鼠伤寒沙门氏菌TA100更敏感，但鼠伤寒沙门氏菌TA100对N-亚硝基环胺的致突变作用更敏感。仓鼠肝脏S9对N-亚硝胺的代谢活化作用优于大鼠肝脏S9，预孵育步骤增强了N-亚硝胺的致突变性。
