Diedrich D L, Fralick J A
J Bacteriol. 1982 Jan;149(1):156-60. doi: 10.1128/jb.149.1.156-160.1982.
Cultures of Escherichia coli K-12 which possessed varied amounts of the LamB protein were found to contain reduced amounts of the OmpC protein. This process was regulated in part at the level of transcription. However, additional controls were inferred from anomalously high levels of the OmpC protein present at low levels of the LamB protein. Both proteins were present at increased levels, and this led to an increase in the total outer membrane protein mass per cell. The absolute amount of outer membrane protein per cell was found not to be a constant as had been tacitly assumed.
研究发现,具有不同量LamB蛋白的大肠杆菌K-12培养物中,OmpC蛋白的含量减少。这一过程部分在转录水平受到调控。然而,从低水平LamB蛋白时OmpC蛋白异常高的水平推断出存在其他调控机制。两种蛋白的水平都升高,这导致每个细胞中外膜蛋白总量增加。结果发现,每个细胞中外膜蛋白的绝对量并非如一直默认的那样是恒定的。