Beltz B, Eisen J S, Flamm R, Harris-Warrick R M, Hooper S L, Marder E
J Exp Biol. 1984 Mar;109:35-54. doi: 10.1242/jeb.109.1.35.
The serotonergic innervation of the stomatogastric ganglion (STG) of three decapod crustacean species, Panulirus interruptus, Homarus americanus and Cancer irroratus, was studied. Immunohistochemical techniques were used to study the distribution of serotonin-like staining in regions of the stomatogastric system in the three species. In C. irroratus and H. americanus, but not in P. interruptus, serotonin-like staining was found in fibres in the stomatogastric nerve and in neuropil regions of the STG. High performance liquid chromatography confirmed the presence of serotonin in STG of C. irroratus and H. americanus, but serotonin was not found in STG of P. interruptus. Electrophysiological experiments showed that the pyloric motor output of the STG of all three species was influenced by bath applications of serotonin. The STG of P. interruptus responded to serotonin concentrations as low as 10-9M; however the STG of the other two species did not respond until serotonin concentrations in excess of 10-6M were applied. We conclude that serotonin may play a hormonal role in the control of the STG of P. interruptus, but is likely to be a neurotransmitter released by inputs to the STG of H. americanus and C. irroratus.
研究了三种十足目甲壳类动物，即断沟龙虾（Panulirus interruptus）、美洲螯龙虾（Homarus americanus）和红斑黄道蟹（Cancer irroratus）的口胃神经节（STG）的5-羟色胺能神经支配。采用免疫组织化学技术研究了这三种动物口胃系统区域中5-羟色胺样染色的分布。在红斑黄道蟹和美洲螯龙虾中，但在断沟龙虾中未发现，在口胃神经的纤维和口胃神经节的神经毡区域中发现了5-羟色胺样染色。高效液相色谱法证实红斑黄道蟹和美洲螯龙虾的口胃神经节中存在5-羟色胺，但在断沟龙虾的口胃神经节中未发现5-羟色胺。电生理实验表明，三种动物口胃神经节的幽门运动输出均受到浴液中5-羟色胺的影响。断沟龙虾的口胃神经节对低至10^-9M的5-羟色胺浓度有反应；然而，其他两种动物的口胃神经节直到应用超过10^-6M的5-羟色胺浓度才会有反应。我们得出结论，5-羟色胺可能在断沟龙虾口胃神经节的控制中起激素作用，但很可能是美洲螯龙虾和红斑黄道蟹口胃神经节的输入释放的神经递质。
