Notario A, Cardenà A, Ricetti M, Pitto P, Trpin L, Barbieri G
Arch Sci Med (Torino). 1978 Jan-Mar;135(1):1-8.
Membrane proteins in circulating blood cells were examined in chick embryos on the 4th, 5th, 6th, 7th and 10th incubation day, in chicks immediately after hatching, and in adult birds. Haemoglobin electrophoresis patterns were determined on cellulose acetate after solubilisation with urea, and in gradient of pH and concentration on acrylamide gels after solubilisation in SDS. Amino acids and other amino compounds were also determined after acid hydrolysis and resin chromatography. Haemoglobin patterns were similar to those displayed by the membrane proteins. The latter, however, changed more slowly.
在孵化第4天、第5天、第6天、第7天和第10天的鸡胚、刚孵化出的雏鸡以及成年鸡中，对循环血细胞中的膜蛋白进行了检测。用尿素溶解后，在醋酸纤维素上测定血红蛋白电泳图谱，在SDS中溶解后，在聚丙烯酰胺凝胶的pH和浓度梯度上测定。酸水解和树脂色谱法后还测定了氨基酸和其他氨基化合物。血红蛋白图谱与膜蛋白所显示的图谱相似。然而，后者的变化更为缓慢。
