Das A, Yanofsky C
Nucleic Acids Res. 1984 Jun 11;12(11):4757-68. doi: 10.1093/nar/12.11.4757.
Expression of trpB and trpA of the Escherichia coli tryptophan operon is shown to be "translationally coupled", i.e., efficient translation of the trpA coding region is dependent on prior translation of the trpB coding region and termination of translation at the trpB stop codon. To examine the dependence of trpA expression on the ribosome binding site sequence in the distal segment of trpB, deletions were produced that replaced this trpB sequence. Analysis of trpA expression in these deletion mutants established that the ribosome binding site sequence is required for efficient translation of the trpA segment of trp mRNA. A modest effect of translation over the trpA ribosome binding site on independent initiation at that site was also observed.
大肠杆菌色氨酸操纵子的trpB和trpA的表达显示为“翻译偶联”，即trpA编码区的有效翻译依赖于trpB编码区的先翻译以及在trpB终止密码子处的翻译终止。为了研究trpA表达对trpB远端片段中核糖体结合位点序列的依赖性，构建了取代该trpB序列的缺失突变体。对这些缺失突变体中trpA表达的分析表明，核糖体结合位点序列是trp mRNA的trpA片段有效翻译所必需的。还观察到在trpA核糖体结合位点上的翻译对该位点独立起始有适度影响。
