Nyholm R E, Currie G A
Br J Cancer. 1978 Mar;37(3):337-44. doi: 10.1038/bjc.1978.52.
Peripheral blood mononuclear cells will lyse antibody-treated human erythrocytes. Using Group A red cells and a hyperimmune anti-A1 serum, we have devised a microassay for the cytolytic capacity of mononuclear cell suspensions. The effector cells responsible for red-cell lysis are mononuclear, adherent and phagocytic, and their activity is blocked by aggregated IgG. Their presence correlates well with non-specific esterase-containing cells and we conclude that they are monocytes. Dose-response curves of red-cell lysis plotted against numbers of monocytes were used to derive a simple parameter expressing the number of monocytes needed to lyse 15% of the 51Cr-labelled red cells. The assay was applied to a group of 27 normal controls and 36 patients with a histologically proven diagnosis of malignant melanoma. The results indicate that monocytes from patients show significantly greater lytic activity than those from the controls. These data suggest that monocytes from cancer patients are in some way activated, and that other defects in monocyte function which have been detected in cancer patients (defective chemotaxis and maturation) may be associated with monocyte "activation".
外周血单个核细胞会溶解经抗体处理的人红细胞。利用A组红细胞和超免疫抗A1血清，我们设计了一种用于检测单核细胞悬液细胞溶解能力的微量测定法。负责红细胞溶解的效应细胞是单核的、贴壁的且具有吞噬作用，其活性被聚集的IgG阻断。它们的存在与含非特异性酯酶的细胞密切相关，我们得出结论，它们是单核细胞。以单核细胞数量为横坐标绘制的红细胞溶解剂量-反应曲线，用于得出一个简单参数，该参数表示溶解15%的51Cr标记红细胞所需的单核细胞数量。该测定法应用于一组27名正常对照者和36名经组织学确诊为恶性黑色素瘤的患者。结果表明，患者的单核细胞表现出比对照者显著更高的溶解活性。这些数据表明，癌症患者的单核细胞在某种程度上被激活，并且在癌症患者中检测到的单核细胞功能的其他缺陷（趋化性和成熟缺陷）可能与单核细胞“激活”有关。
