从T淋巴细胞中进一步纯化中性粒细胞迁移抑制因子（NIF-T）：NIF-T与白细胞抑制因子（LIF）在免疫学上不同的证据

Further purification of neutrophil migration inhibition factor from T lymphocytes (NIF-T): evidence that NIF-T and leukocyte inhibitory factor (LIF) are immunologically distinct.

作者信息

Weisbart R H, Chan G, Spolter L, Golde D W

出版信息

Clin Immunol Immunopathol. 1984 Sep;32(3):269-74. doi: 10.1016/0090-1229(84)90271-x.

Abstract

Neutrophil migration inhibition factor from T lymphocytes (NIF-T) purified by antibody affinity chromatography and gel filtration chromatography was radioiodinated and identified as a 26,000-MW protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). NIF-T was identified by elution of biological activity from gel fractions and selective adsorption of a radioiodinated mediator by HL60 cells differentiated in the presence of dimethyl sulfoxide (DMSO) to develop receptors for NIF-T. A goat neutralizing antibody for NIF-T neutralized and immunoprecipitated migration inhibition activity in the conditioned medium from Mo T-lymphoblast cells and human peripheral blood lymphocytes (PBL) cultured with concanavalin A (Con A), but not from RPMI 1788 B-lymphoblast cells with leukocyte migration inhibitory factor (LIF) activity. These studies distinguish NIF-T both chemically and immunologically from LIF.

摘要

通过抗体亲和层析和凝胶过滤层析纯化的来自T淋巴细胞的中性粒细胞迁移抑制因子（NIF-T）进行了放射性碘化，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）鉴定为一种分子量为26,000的蛋白质。通过从凝胶组分中洗脱生物活性以及在二甲基亚砜（DMSO）存在下分化以产生NIF-T受体的HL60细胞对放射性碘化介质的选择性吸附来鉴定NIF-T。针对NIF-T的山羊中和抗体可中和并免疫沉淀来自用刀豆球蛋白A（Con A）培养的Mo T淋巴细胞和成人人外周血淋巴细胞（PBL）的条件培养基中的迁移抑制活性，但不能中和来自具有白细胞迁移抑制因子（LIF）活性的RPMI 1788 B淋巴细胞的条件培养基中的迁移抑制活性。这些研究在化学和免疫学上区分了NIF-T和LIF。